S. Shkurdoda, D. Shynkarenko, V. Pasichnyk, K. Korol, O. Posilskyi
The article deals with the recommendations for the detection of isoniazid in meat products, using the example of minced meat using physicochemical methods. This study does not lose its relevance over the years, due to the use of drugs for tuberculosis for poisoning homeless and domestic animals, in particular dogs, in different regions of Ukraine, the main active ingredient of which is isoniazid. The article describes a method for the extraction of isoniazid in food products of meat origin (minced meat, sausage), which presumably could or may be used to poison domestic animals, in particular dogs. Isoniazid was identified by the methods of qualitative color reactions, thin-layer chromatography, and gas chromatography with a mass-selective detector.
The process of qualitative identification of isoniazid in foodstuffs that could or may be used to poison domestic animals, in particular dogs, includes:
-the method of extraction and separation of interfering impurities, primarily fats, preliminary research by the method of qualitative analytical reactions;
-studies by thin layer chromatography;
-research by instrumental methods, in particular, gas chromatography with mass-selective detection.
The method for extracting isoniazid from meat products described in the article is effective and allows you to get rid of interfering substances, especially fats. In addition, the method of gas chromatography with mass-selective detection has sufficient sensitivity and selectivity. It allows with a minimum number of interfering peaks of low intensity, determine the composition of isoniazid accurately.
The specified list of instrumental base, methods and research techniques is not exhaustive. The examiner should be guided by the availability of relevant literature and the availability of appropriate analytical equipment (gas chromatograph, gas chromatograph with mass-selective detector, liquid chromatograph, liquid chromatograph with mass-selective detector, etc.). In accordance with this, it is necessary to use certain methods for studying substances. These methods are subject to mandatory verification before being used in the laboratory.
The obtained research results allow us to recommend this technique for implementation in expert practice.
Keywords: poisoning of domestic animals, drug, isoniazid, qualitative chemical reactions, thin-layer chromatography, gas, chromatography-mass spectrometry, forensic examination.